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Disulfide Trapping for Modeling and Structure Determination of Receptor:Chemokine Complexes

Despite the recent breakthrough advances in GPCR crystallography, structure determination of protein-protein complexes involving chemokine receptors and their endogenous chemokine ligands remains challenging. Here we describe disulfide trapping, a methodology for generating irreversible covalent bin...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Main Authors: Kufareva, Irina, Gustavsson, Martin, Holden, Lauren G., Qin, Ling, Zheng, Yi, Handel, Tracy M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4782801/
https://ncbi.nlm.nih.gov/pubmed/26921956
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2015.12.001
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