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A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro

Incorporation of selenocysteine (Sec) in bacteria requires a UGA codon that is reassigned to Sec by the Sec-specific elongation factor SelB and a conserved mRNA motif (SECIS element). These requirements severely restrict the engineering of selenoproteins. Earlier a synthetic tRNA(Sec) was reported t...

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Pubblicato in:FEBS Lett
Autori principali: Miller, Corwin, Bröcker, Markus J., Prat, Laure, Ip, Kevan, Chirathivat, Napon, Feiock, Alexander, Veszprémi, Miklós, Söll, Dieter
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4782793/
https://ncbi.nlm.nih.gov/pubmed/26160755
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.febslet.2015.06.039
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