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A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro
Incorporation of selenocysteine (Sec) in bacteria requires a UGA codon that is reassigned to Sec by the Sec-specific elongation factor SelB and a conserved mRNA motif (SECIS element). These requirements severely restrict the engineering of selenoproteins. Earlier a synthetic tRNA(Sec) was reported t...
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| Publicado no: | FEBS Lett |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4782793/ https://ncbi.nlm.nih.gov/pubmed/26160755 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.febslet.2015.06.039 |
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