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Single-Molecule Imaging in Living Drosophila Embryos with Reflected Light-Sheet Microscopy

In multicellular organisms, single-fluorophore imaging is obstructed by high background. To achieve a signal/noise ratio conducive to single-molecule imaging, we adapted reflected light-sheet microscopy (RLSM) to image highly opaque late-stage Drosophila embryos. Alignment steps were modified by mea...

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Vydáno v:Biophys J
Hlavní autoři: Greiss, Ferdinand, Deligiannaki, Myrto, Jung, Christophe, Gaul, Ulrike, Braun, Dieter
Médium: Artigo
Jazyk:Inglês
Vydáno: The Biophysical Society 2016
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4776035/
https://ncbi.nlm.nih.gov/pubmed/26910430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.12.035
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