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Single-Molecule Imaging in Living Drosophila Embryos with Reflected Light-Sheet Microscopy
In multicellular organisms, single-fluorophore imaging is obstructed by high background. To achieve a signal/noise ratio conducive to single-molecule imaging, we adapted reflected light-sheet microscopy (RLSM) to image highly opaque late-stage Drosophila embryos. Alignment steps were modified by mea...
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| 出版年: | Biophys J |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
The Biophysical Society
2016
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4776035/ https://ncbi.nlm.nih.gov/pubmed/26910430 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.12.035 |
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