Easy mammalian expression and crystallography of maltose-binding protein-fused human proteins

We present a strategy to obtain milligrams of highly post-translationally modified eukaryotic proteins, transiently expressed in mammalian cells as rigid or cleavable fusions with a mammalianized version of bacterial maltose-binding protein (mMBP). This variant was engineered to combine mutations th...

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Detalhes bibliográficos
Publicado no:J Struct Biol
Main Authors: Bokhove, Marcel, Sadat Al Hosseini, Hamed, Saito, Takako, Dioguardi, Elisa, Gegenschatz-Schmid, Katharina, Nishimura, Kaoru, Raj, Isha, de Sanctis, Daniele, Han, Ling, Jovine, Luca
Formato: Artigo
Idioma:Inglês
Publicado em: Academic Press 2016
Assuntos:
Technical Note
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4771870/
https://ncbi.nlm.nih.gov/pubmed/26850170
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2016.01.016
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