Secretion and Proteolysis of Heterologous Proteins Fused to the Escherichia coli Maltose Binding Protein in Pichia pastoris

The E. coli maltose binding protein (MBP) has been utilized as a translational fusion partner to improve the expression of foreign proteins made in E. coli. When located N-terminal to its cargo protein, MBP increases the solubility of intracellular proteins and improves the export of secreted protei...

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Detalhes bibliográficos
Main Authors: Li, Zhiguo, Leung, Wilson, Yon, Amy, Nguyen, John, Perez, Vincent C., Vu, Jane, Giang, William, Luong, Linda T., Phan, Tracy, Salazar, Katherine A., Gomez, Seth R., Au, Colin, Xiang, Fan, Thomas, David W., Franz, Andreas H., Lin-Cereghino, Joan, Lin-Cereghino, Geoff P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2860017/
https://ncbi.nlm.nih.gov/pubmed/20230898
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2010.03.004
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