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Enhancing the selective extracellular location of a recombinant E. coli domain antibody by management of fermentation conditions
The preparation of a recombinant protein using Escherichia coli often involves a challenging primary recovery sequence. This is due to the inability to secrete the protein to the extracellular space without a significant degree of cell lysis. This results in the release of nucleic acids, leading to...
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| Publicado no: | Appl Microbiol Biotechnol |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer Berlin Heidelberg
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4768232/ https://ncbi.nlm.nih.gov/pubmed/26184976 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-015-6799-3 |
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