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Expression and purification of orphan cytochrome P450 4X1 and oxidation of anandamide
Cytochrome P450 (P450) 4X1 is one of the so-called “orphan” P450s without assigned biological function. Codon-optimized P450 4X1 and a number of N-terminal modified sequences were expressed in Escherichia coli. Native P450 4X1 showed a characteristic P450 spectrum but low expression in E. coli DH5α...
में बचाया:
| में प्रकाशित: | FEBS J |
|---|---|
| मुख्य लेखकों: | , , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
2008
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4765179/ https://ncbi.nlm.nih.gov/pubmed/18549450 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1742-4658.2008.06518.x |
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