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Sample preparation protocol for bottom-up proteomic analysis of the secretome of the islets of Langerhans
We evaluate a set of protocols for preparation of the secretome from murine Islets of Langerhans for bottom-up proteomic analysis. Of the protocols evaluated, a filter-aided sample preparation based approach using sodium dodecyl sulfate as a detergent to solubilize proteins generated the most protei...
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| Publicado no: | Analyst |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4764456/ https://ncbi.nlm.nih.gov/pubmed/26863548 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c5an02265g |
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