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HBx-DNA probe preparation and its application in study of hepatocarcinogenesis

AIM: To study the role of HBV especially HBx Open Reading Frame (ORF) in the development of hepatocellular carcinoma (HCC). METHODS: HBV 3.2 kb fragment was retrieved by digesting recombinant plasmid pBR322-2HBV with EcoR I, and HBx 0.59 kb fragments by digesting HBV-DNA with BamH I and Bgl II. Thes...

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Detalhes bibliográficos
Publicado no:World J Gastroenterol
Main Authors: Gao, Feng-Guang, Sun, Wen-Sheng, Cao, Ying-Lin, Zhang, Li-Ning, Song, Jing, Li, Hua-Fen, Yan, Shi-Kun
Formato: Artigo
Idioma:Inglês
Publicado em: Baishideng Publishing Group Inc 1998
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4761550/
https://ncbi.nlm.nih.gov/pubmed/11819309
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v4.i4.320
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