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Optimization of the Cytokine Secretion Assay for human IL-2 in single and combination assays

The cytokine secretion assay identifies live cytokine-secreting cells by capturing the secreted cytokine on a surface-bound capture antibody in dilute suspension culture, followed by detection with a fluorescent anti-cytokine antibody. However, examining the kinetics of cytokine detection revealed t...

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Detalhes bibliográficos
Publicado no:Cytometry A
Main Authors: Deng, Nan, Mosmann, Tim R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4759652/
https://ncbi.nlm.nih.gov/pubmed/25919308
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cyto.a.22668
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