Automated analysis of two- and three-color fluorescent Elispot (Fluorospot) assays for cytokine secretion

The Elispot effectively measures the frequencies of cells secreting particular molecules, especially low-frequency cells such as antigen-specific T cells. The Fluorospot assay adapted this analysis to two products per cell, and this has now been extended to three-color measurement of both mouse and...

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Udgivet i:Comput Methods Programs Biomed
Main Authors: Rebhahn, Jonathan A., Bishop, Courtney, Divekar, Anagha A., Jiminez-Garcia, Katty, Kobie, James J., Lee, F. Eun-Hyung, Maupin, Genny M., Snyder-Cappione, Jennifer E., Zaiss, Dietmar M., Mosmann, Tim R.
Format: Artigo
Sprog:Inglês
Udgivet: 2008
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4440339/
https://ncbi.nlm.nih.gov/pubmed/18644656
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cmpb.2008.06.002
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