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Mathematical Model of the Firefly Luciferase Complementation Assay Reveals a Non-Linear Relationship between the Detected Luminescence and the Affinity of the Protein Pair Being Analyzed

The firefly luciferase complementation assay is widely used as a bioluminescent reporter technology to detect protein-protein interactions in vitro, in cellulo, and in vivo. Upon the interaction of a protein pair, complemented firefly luciferase emits light through the adenylation and oxidation of i...

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Библиографические подробности
Опубликовано в: :PLoS One
Главные авторы: Dale, Renee, Ohmuro-Matsuyama, Yuki, Ueda, Hiroshi, Kato, Naohiro
Формат: Artigo
Язык:Inglês
Опубликовано: Public Library of Science 2016
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4757408/
https://ncbi.nlm.nih.gov/pubmed/26886551
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0148256
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