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Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments
BACKGROUND: Human interactome is predicted to contain 150,000 to 300,000 protein-protein interactions, (PPIs). Protein-fragment complementation assay (PCA) is one of the most widely used methods to detect PPI, as well as Förster resonance energy transfer (FRET). To date, successful applications of f...
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
BioMed Central
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3626928/ https://ncbi.nlm.nih.gov/pubmed/23536995 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-31 |
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