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An efficient protocol for isolation of inhibitor-free nucleic acids even from recalcitrant plants

For fast and easy isolation of inhibitor-free genomic DNA even from the toughest plant leaf samples, including those high in polyphenols and polysaccharides, a protocol has been developed. To prevent the solubility of polysaccharides in the DNA extract, high salt concentration (1.4 M) was used in th...

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Publicat a:3 Biotech
Autors principals: Rezadoost, Mohammad Hossein, Kordrostami, Mojtaba, Kumleh, Hassan Hassani
Format: Artigo
Idioma:Inglês
Publicat: Springer Berlin Heidelberg 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4752943/
https://ncbi.nlm.nih.gov/pubmed/28330131
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-016-0375-0
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