A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing

BACKGROUND: Woody tropical plants contain high levels of complex organic compounds that inhibit the chemical procedures needed to extract RNA or DNA, thus compromising downstream applications such as RNA sequencing and analysis of gene expression. To overcome this issue, researchers must use extract...

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Pubblicato in:Plant Methods
Autori principali: Barbier, François F., Chabikwa, Tinashe G., Ahsan, Muhammad U., Cook, Stacey E., Powell, Rosanna, Tanurdzic, Milos, Beveridge, Christine A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2019
Soggetti:
Methodology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6549277/
https://ncbi.nlm.nih.gov/pubmed/31171930
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-019-0447-3
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