A genetic method for defining DNA-binding domains: application to the nuclear receptor NGFI-B.
A method is described that allows for rapid and efficient generation of functional mutations in DNA-binding domains of proteins. The target DNA-binding domain is attached to the Gal4p transcriptional-activating domain and expressed in yeast. The binding site recognized by the target domain is placed...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1993
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC47527/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8415675/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.19.9186 |
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