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A genetic method for defining DNA-binding domains: application to the nuclear receptor NGFI-B.

A method is described that allows for rapid and efficient generation of functional mutations in DNA-binding domains of proteins. The target DNA-binding domain is attached to the Gal4p transcriptional-activating domain and expressed in yeast. The binding site recognized by the target domain is placed...

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Dettagli Bibliografici
Autori principali: Wilson, T E, Padgett, K A, Johnston, M, Milbrandt, J
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1993
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC47527/
https://ncbi.nlm.nih.gov/pubmed/8415675
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