Processing of a fusion protein by endoprotease in COS-1 cells for secretion of mature peptide by using a chimeric expression vector.

The subtilisin-related proprotein convertase furin is expressed in various mammalian tissues. Expecting that COS-1 cells have a furin-like endoprotease, we constructed a fusion expression vector for production of a recombinant foreign protein having no signal peptide or a protein in truncated form i...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Liu, Y C, Kawagishi, M, Mikayama, T, Inagaki, Y, Takeuchi, T, Ohashi, H
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1993
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC47480/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8415638/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.19.8957
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