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In-membrane chemical modification (IMCM) for site-specific chromophore labeling of GPCRs
We present “in-membrane chemical modification” (IMCM) for obtaining selective chromophore labeling of intracellular surface cysteines in G protein-coupled receptors (GPCRs) with minimal mutagenesis. Practical use of IMCM is illustrated with site-specific introduction of chromophores for NMR and fluo...
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| Izdano u: | Angew Chem Int Ed Engl |
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| Glavni autori: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2015
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4747426/ https://ncbi.nlm.nih.gov/pubmed/26545333 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201508506 |
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