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A continuous spectrophotometric enzyme-coupled assay for deoxynucleoside triphosphate triphosphohydrolases

We describe here a continuous, spectrophotometric, enzyme-coupled assay useful to monitor reactions catalyzed by nucleoside triphosphohydrolases. In particular, using Escherichia coli deoxynucleoside triphosphohydrolase (Dgt), which hydrolyzes dGTP to deoxyguanosine and tripolyphosphate (PPP(i)), as...

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Publicat a:Anal Biochem
Autors principals: Singh, Deepa, Schaaper, Roel M., Hochkoeppler, Alejandro
Format: Artigo
Idioma:Inglês
Publicat: 2015
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4744544/
https://ncbi.nlm.nih.gov/pubmed/26723493
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.11.027
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