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A continuous spectrophotometric enzyme-coupled assay for deoxynucleoside triphosphate triphosphohydrolases
We describe here a continuous, spectrophotometric, enzyme-coupled assay useful to monitor reactions catalyzed by nucleoside triphosphohydrolases. In particular, using Escherichia coli deoxynucleoside triphosphohydrolase (Dgt), which hydrolyzes dGTP to deoxyguanosine and tripolyphosphate (PPP(i)), as...
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| Publicado no: | Anal Biochem |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4744544/ https://ncbi.nlm.nih.gov/pubmed/26723493 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.11.027 |
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