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A flow-cytometry-based method to simplify the analysis and quantification of protein association to chromatin in mammalian cells

Protein accumulation on chromatin has traditionally been studied using immunofluorescence microscopy or biochemical cellular fractionation followed by western immunoblot analysis. As a way to improve the reproducibility of this kind of analysis, make it easier to quantify and allow a stream-lined ap...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Forment, Josep V., Jackson, Stephen P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4743064/
https://ncbi.nlm.nih.gov/pubmed/26226461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.066
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