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Dynamic quantification of antigen molecules with flow cytometry
Traditional methods for estimating the number of expressed molecules, based on the detection of target antigens bound with fluorescently labeled antibodies, assume that the antigen-antibody reaction reaches equilibrium. A calibration procedure is used to convert the intensity of the fluorescence sig...
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Publicado no: | J Immunol Methods |
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Main Authors: | , , , , , , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2015
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4380687/ https://ncbi.nlm.nih.gov/pubmed/25687877 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2015.02.001 |
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