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Dynamic quantification of antigen molecules with flow cytometry

Traditional methods for estimating the number of expressed molecules, based on the detection of target antigens bound with fluorescently labeled antibodies, assume that the antigen-antibody reaction reaches equilibrium. A calibration procedure is used to convert the intensity of the fluorescence sig...

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Vydáno v:	J Immunol Methods
Hlavní autoři:	Moskalensky, A.E., Chernyshev, A.V., Yurkin, M.A., Nekrasov, V.M., Polshchitsin, A.A., Parks, D.R., Moore, W.A., Herzenberg, L.A., Filatenkov, A., Maltsev, V.P., Orlova, D.Y.
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	2015
Témata:	Article
On-line přístup:	https://ncbi.nlm.nih.gov/pmc/articles/PMC4380687/ https://ncbi.nlm.nih.gov/pubmed/25687877 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2015.02.001
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Dynamic quantification of antigen molecules with flow cytometry

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