Immobilized Metal Affinity Chromatography Coupled to Multiple Reaction Monitoring Enables Reproducible Quantification of Phospho-signaling

A major goal in cell signaling research is the quantification of phosphorylation pharmacodynamics following perturbations. Traditional methods of studying cellular phospho-signaling measure one analyte at a time with poor standardization, rendering them inadequate for interrogating network biology a...

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Detalhes bibliográficos
Publicado no:Mol Cell Proteomics
Main Authors: Kennedy, Jacob J., Yan, Ping, Zhao, Lei, Ivey, Richard G., Voytovich, Uliana J., Moore, Heather D., Lin, Chenwei, Pogosova-Agadjanyan, Era L., Stirewalt, Derek L., Reding, Kerryn W., Whiteaker, Jeffrey R., Paulovich, Amanda G.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2016
Assuntos:
Technological Innovation and Resources
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4739685/
https://ncbi.nlm.nih.gov/pubmed/26621847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O115.054940
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