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Peptide Immunoaffinity Enrichment and Targeted Mass Spectrometry Enables Multiplex, Quantitative Pharmacodynamic Studies of Phospho-Signaling

In most cell signaling experiments, analytes are measured one Western blot lane at a time in a semiquantitative and often poorly specific manner, limiting our understanding of network biology and hindering the translation of novel therapeutics and diagnostics. We show the feasibility of using multip...

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Bibliografiske detaljer
Udgivet i:Mol Cell Proteomics
Main Authors: Whiteaker, Jeffrey R., Zhao, Lei, Yan, Ping, Ivey, Richard G., Voytovich, Uliana J., Moore, Heather D., Lin, Chenwei, Paulovich, Amanda G.
Format: Artigo
Sprog:Inglês
Udgivet: The American Society for Biochemistry and Molecular Biology 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4528251/
https://ncbi.nlm.nih.gov/pubmed/25987412
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O115.050351
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