Serum apolipoprotein A-1 quantification by LC–MS with a SILAC internal standard reveals reduced levels in smokers

BACKGROUND: Absolute quantification of protein biomarkers such as serum apolipoprotein A1 by both immunoassays and LC–MS can provide misleading results. RESULTS: Recombinant ApoA-1 internal standard was prepared using stable isotope labeling by amino acids in cell culture with [(13)C(6)(15)N(2)]-lys...

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Detalhes bibliográficos
Publicado no:Bioanalysis
Main Authors: Wang, Qingqing, Zhang, Suhong, Guo, Lili, Busch, Christine M, Jian, Wenying, Weng, Naidong, Snyder, Nathaniel W, Rangiah, Kannan, Mesaros, Clementina, Blair, Ian A
Formato: Artigo
Idioma:Inglês
Publicado em: Future Science Ltd 2015
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4737526/
https://ncbi.nlm.nih.gov/pubmed/26394123
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4155/bio.15.195
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