Production of high-titer helper-free retroviruses by transient transfection.

The generation of high-titer, helper-free retroviruses by transient transfection has been achieved by using the highly transfectable 293T cell line into which are stably introduced constructs that express retroviral packaging functions. The resulting ecotropic virus packaging cell line BOSC 23 produ...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Pear, W S, Nolan, G P, Scott, M L, Baltimore, D
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1993
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC47362/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7690960/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.18.8392
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