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Production of high-titer helper-free retroviruses by transient transfection.

The generation of high-titer, helper-free retroviruses by transient transfection has been achieved by using the highly transfectable 293T cell line into which are stably introduced constructs that express retroviral packaging functions. The resulting ecotropic virus packaging cell line BOSC 23 produ...

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Detalhes bibliográficos
Main Authors: Pear, W S, Nolan, G P, Scott, M L, Baltimore, D
Formato: Artigo
Idioma:Inglês
Publicado em: 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC47362/
https://ncbi.nlm.nih.gov/pubmed/7690960
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