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Effects of CreER(T2), 4-OH Tamoxifen, and Gender on CFU-F Assays

Gene function in stem cell maintenance is often tested by inducing deletion via the Cre-loxP system. However, controls for Cre and other variables are frequently not included. Here we show that when cultured in the presence of 4-OH tamoxifen, bone and marrow cells containing the CreER(T2) construct...

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Pubblicato in:PLoS One
Autori principali: McHaffie, Sophie L., Hastie, Nicholas D., Chau, You-Ying
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4734617/
https://ncbi.nlm.nih.gov/pubmed/26828722
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0148105
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