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Simple horizontal magnetic tweezers for micromanipulation of single DNA molecules and DNA–protein complexes

We report the development of a simple-to-implement magnetic force transducer that can apply a wide range of piconewton (pN) scale forces on single DNA molecules and DNA–protein complexes in the horizontal plane. The resulting low-noise force-extension data enable very high-resolution detection of ch...

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Détails bibliographiques
Publié dans:Biotechniques
Auteurs principaux: McAndrew, Christopher P., Tyson, Christopher, Zischkau, Joseph, Mehl, Patrick, Tuma, Pamela L., Pegg, Ian L., Sarkar, Abhijit
Format: Artigo
Langue:Inglês
Publié: 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4733873/
https://ncbi.nlm.nih.gov/pubmed/26757808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114369
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