CRISPRi-Manipulation of Genetic Code Expansion via RF1 for Reassignment of Amber Codon in Bacteria

The precise engineering of proteins in bacteria via the amber codon has been hampered by the poor incorporation of unnatural amino acid (UAA). Here we explored the amber assignment as a sense codon for UAA by CRISPRi targeting release factor 1 (RF1). Scanning of RF1 gene with sgRNAs identified targe...

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Bibliographic Details
Published in:Sci Rep
Main Authors: Zhang, Bo, Yang, Qi, Chen, Jingxian, Wu, Ling, Yao, Tianzhuo, Wu, Yiming, Xu, Huan, Zhang, Lihe, Xia, Qing, Zhou, Demin
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 2016
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4730227/
https://ncbi.nlm.nih.gov/pubmed/26818534
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep20000
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