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Application of restriction display PCR technique in the preparation of cDNA microarray probes

AIM: To develop a simplified and efficient method for the preparation of hepatitis C virus (HCV) cDNA microarray probes. METHODS: With the technique of restriction display PCR (RD-PCR), restriction enzyme Sau3A I was chosen to digest the full-length HCV cDNAs. The products were classified and re-amp...

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Detalhes bibliográficos
Publicado no:World J Gastroenterol
Main Authors: Sun, Zhao-Hui, Ma, Wen-Li, Zhang, Bao, Peng, Yi-Fei, Zheng, Wen-Ling
Formato: Artigo
Idioma:Inglês
Publicado em: Baishideng Publishing Group Inc 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4727234/
https://ncbi.nlm.nih.gov/pubmed/16437681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i48.7579
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