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Detection of hepatitis B virus DNA by real-time PCR using TaqMan-MGB probe technology

AIM: To develop a real-time PCR for detecting hepatitis B virus (HBV) DNA based on TaqMan technology using a new MGB probe. METHODS: Plasmid containing the sequence of X gene (1414-1744 nt) was constructed as HBV-DNA standard for quantitative analysis. A TaqMan-MGB probe between primers for amplific...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:World J Gastroenterol
Hauptverfasser: Zhao, Jin-Rong, Bai, Yu-Jie, Zhang, Qing-Hua, Wan, Yan, Li, Ding, Yan, Xiao-Jun
Format: Artigo
Sprache:Inglês
Veröffentlicht: Baishideng Publishing Group Inc 2005
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4250800/
https://ncbi.nlm.nih.gov/pubmed/15641135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i4.508
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