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A practical method for monitoring FRET-based biosensors in living animals using two-photon microscopy

The commercial availability of multiphoton microscope systems has nurtured the growth of intravital microscopy as a powerful technique for evaluating cell biology in the relevant context of living animals. In parallel, new fluorescent protein (FP) biosensors have become available that enable studies...

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Veröffentlicht in:Am J Physiol Cell Physiol
Hauptverfasser: Tao, Wen, Rubart, Michael, Ryan, Jennifer, Xiao, Xiao, Qiao, Chunping, Hato, Takashi, Davidson, Michael W., Dunn, Kenneth W., Day, Richard N.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Physiological Society 2015
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4725439/
https://ncbi.nlm.nih.gov/pubmed/26333599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajpcell.00182.2015
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