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Direct Image-Based Enumeration of Clostridium phytofermentans Cells on Insoluble Plant Biomass Growth Substrates

A dual-fluorescent-dye protocol to visualize and quantify Clostridium phytofermentans ISDg (ATCC 700394) cells growing on insoluble cellulosic substrates was developed by combining calcofluor white staining of the growth substrate with cell staining using the nucleic acid dye Syto 9. Cell growth, ce...

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Bibliografische gegevens
Gepubliceerd in:Appl Environ Microbiol
Hoofdauteurs: Alvelo-Maurosa, Jesús G., Lee, Scott J., Hazen, Samuel P., Leschine, Susan B.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology 2016
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4725282/
https://ncbi.nlm.nih.gov/pubmed/26637592
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03119-15
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