Direct Image-Based Enumeration of Clostridium phytofermentans Cells on Insoluble Plant Biomass Growth Substrates

A dual-fluorescent-dye protocol to visualize and quantify Clostridium phytofermentans ISDg (ATCC 700394) cells growing on insoluble cellulosic substrates was developed by combining calcofluor white staining of the growth substrate with cell staining using the nucleic acid dye Syto 9. Cell growth, ce...

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Bibliographic Details
Published in:Appl Environ Microbiol
Main Authors: Alvelo-Maurosa, Jesús G., Lee, Scott J., Hazen, Samuel P., Leschine, Susan B.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2016
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Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4725282/
https://ncbi.nlm.nih.gov/pubmed/26637592
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03119-15
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