Characterization of a Mannose-6-Phosphate Isomerase from Bacillus amyloliquefaciens and Its Application in Fructose-6-Phosphate Production

The BaM6PI gene encoding a mannose-6-phosphate isomerase (M6PI, EC 5.3.1.8) was cloned from Bacillus amyloliquefaciens DSM7 and overexpressed in Escherichia coli. The enzyme activity of BaM6PI was optimal at pH and temperature of 7.5 and 70°C, respectively, with a k(cat)/K(m) of 13,900 s(-1) mM(-1)...

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Udgivet i:PLoS One
Main Authors: Sigdel, Sujan, Singh, Ranjitha, Kim, Tae-Su, Li, Jinglin, Kim, Sang-Yong, Kim, In-Won, Jung, Woo-Suk, Pan, Cheol-Ho, Kang, Yun Chan, Lee, Jung-Kul
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2015
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4718643/
https://ncbi.nlm.nih.gov/pubmed/26171785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0131585
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