Characterization of a Mannose-6-Phosphate Isomerase from Bacillus amyloliquefaciens and Its Application in Fructose-6-Phosphate Production

The BaM6PI gene encoding a mannose-6-phosphate isomerase (M6PI, EC 5.3.1.8) was cloned from Bacillus amyloliquefaciens DSM7 and overexpressed in Escherichia coli. The enzyme activity of BaM6PI was optimal at pH and temperature of 7.5 and 70°C, respectively, with a k(cat)/K(m) of 13,900 s(-1) mM(-1)...

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Bibliografiska uppgifter
I publikationen:PLoS One
Huvudupphovsmän: Sigdel, Sujan, Singh, Ranjitha, Kim, Tae-Su, Li, Jinglin, Kim, Sang-Yong, Kim, In-Won, Jung, Woo-Suk, Pan, Cheol-Ho, Kang, Yun Chan, Lee, Jung-Kul
Materialtyp: Artigo
Språk:Inglês
Publicerad: Public Library of Science 2015
Ämnen:
Research Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4718643/
https://ncbi.nlm.nih.gov/pubmed/26171785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0131585
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