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Induction of pancreatic duct cells of neonatal rats into insulin-producing cells with fetal bovine serum: A natural protocol and its use for patch clamp experiments

AIM: To induce the pancreatic duct cells into endocrine cells with a new natural protocol for electrophysiological study. METHODS: The pancreatic duct cells of neonatal rats were isolated, cultured and induced into endocrine cells with 15% fetal bovine serum for a period of 20 d. During this period,...

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Publicat a:World J Gastroenterol
Autors principals: Leng, San-Hua, Lu, Fu-Er
Format: Artigo
Idioma:Inglês
Publicat: Baishideng Publishing Group Inc 2005
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4717039/
https://ncbi.nlm.nih.gov/pubmed/16437601
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i44.6968
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