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Deconvolution method for specific and nonspecific binding of ligand to multi-protein complex by native mass spectrometry

In native mass spectrometry, it has been difficult to discriminate between specific binding of a ligand to a multi-protein complex from the nonspecific interactions. Here, we present a deconvolution model that consists of two levels of data reduction. At the first level, the apparent association bin...

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Detalhes bibliográficos
Publicado no:Anal Chem
Main Authors: Guan, Shenheng, Trnka, Michael J., Burlingame, Alma L., Bushnell, David A., Robinson, Philip J. J., Kornberg, Roger D., Gestwicki, Jason E., Prusiner, Stanley B.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4714924/
https://ncbi.nlm.nih.gov/pubmed/26189511
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.5b02258
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