Generation of Knock-In Pigs Carrying Oct4-tdTomato Reporter through CRISPR/Cas9-Mediated Genome Engineering

The porcine pluripotent cells that can generate germline chimeras have not been developed. The Oct4 promoter-based fluorescent reporter system, which can be used to monitor pluripotency, is an important tool to generate authentic porcine pluripotent cells. In this study, we established a porcine Oct...

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Vydáno v:PLoS One
Hlavní autoři: Lai, Sisi, Wei, Shu, Zhao, Bentian, Ouyang, Zhen, Zhang, Quanjun, Fan, Nana, Liu, Zhaoming, Zhao, Yu, Yan, Quanmei, Zhou, Xiaoqing, Li, Li, Xin, Jige, Zeng, Yangzhi, Lai, Liangxue, Zou, Qingjian
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2016
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4710570/
https://ncbi.nlm.nih.gov/pubmed/26756580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0146562
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