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Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Structural determination of proteins is rather challenging for proteins with molecular masses between 40 - 200 kDa. Considering that more than half of natural proteins have a molecular mass between 40 - 200 kDa(1,2), a robust and high-throughput method with a nanometer resolution capability is neede...

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Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:J Vis Exp
Asıl Yazarlar: Rames, Matthew, Yu, Yadong, Ren, Gang
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MyJove Corporation 2014
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4710468/
https://ncbi.nlm.nih.gov/pubmed/25145703
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51087
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