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Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
Structural determination of proteins is rather challenging for proteins with molecular masses between 40 - 200 kDa. Considering that more than half of natural proteins have a molecular mass between 40 - 200 kDa(1,2), a robust and high-throughput method with a nanometer resolution capability is neede...
Uloženo v:
| Vydáno v: | J Vis Exp |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
MyJove Corporation
2014
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4710468/ https://ncbi.nlm.nih.gov/pubmed/25145703 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51087 |
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