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Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
Structural determination of proteins is rather challenging for proteins with molecular masses between 40 - 200 kDa. Considering that more than half of natural proteins have a molecular mass between 40 - 200 kDa(1,2), a robust and high-throughput method with a nanometer resolution capability is neede...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4710468/ https://ncbi.nlm.nih.gov/pubmed/25145703 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51087 |
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