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Improved purification, crystallization and crystallographic study of Hyd-2-type [NiFe]-hydrogenase from Citrobacter sp. S-77
The purification procedure of Hyd-2-type [NiFe]-hydrogenase from Citrobacter sp. S-77 was improved by applying treatment with trypsin before chromatography. Purified protein samples both with and without trypsin treatment were successfully crystallized using the sitting-drop vapour-diffusion method...
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| 發表在: | Acta Crystallogr F Struct Biol Commun |
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| Main Authors: | , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
International Union of Crystallography
2016
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4708051/ https://ncbi.nlm.nih.gov/pubmed/26750485 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X15024152 |
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