Improved purification, crystallization and crystallographic study of Hyd-2-type [NiFe]-hydrogenase from Citrobacter sp. S-77

The purification procedure of Hyd-2-type [NiFe]-hydrogenase from Citrobacter sp. S-77 was improved by applying treatment with trypsin before chromatography. Purified protein samples both with and without trypsin treatment were successfully crystallized using the sitting-drop vapour-diffusion method...

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Detalhes bibliográficos
Publicado no:Acta Crystallogr F Struct Biol Commun
Main Authors: Muhd Noor, Noor Dina, Nishikawa, Koji, Nishihara, Hirofumi, Yoon, Ki-Seok, Ogo, Seiji, Higuchi, Yoshiki
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2016
Assuntos:
Research Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4708051/
https://ncbi.nlm.nih.gov/pubmed/26750485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X15024152
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