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Genetic fusion of subunits of a dimeric protein substantially enhances its stability and rate of folding.

The gene V protein of bacteriophage f1 is a single-stranded DNA and RNA-binding protein composed of two identical subunits. We have constructed single-chain variants of the protein using short peptide linkers of five or six amino acids to connect the carboxyl terminus of one monomer to the amino ter...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Liang, H, Sandberg, W S, Terwilliger, T C
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC47065/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8346210/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.15.7010
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