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Dominant lethality by expression of a catalytically inactive class I tRNA synthetase.

Alignment-guided mutagenesis was used to create an inactive, but toxic, aminoacyl-tRNA synthetase. An Asp-96-->Ala (D96A) replacement in the nucleotide binding fold of the class I Escherichia coli isoleucyl-tRNA synthetase inactivates the enzyme without disrupting its competence for binding isole...

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Bibliografiske detaljer
Main Authors: Schmidt, E, Schimmel, P
Format: Artigo
Sprog:Inglês
Udgivet: 1993
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC47046/
https://ncbi.nlm.nih.gov/pubmed/8346197
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