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Design of protein switches based on an ensemble model of allostery

Switchable proteins that can be regulated through exogenous or endogenous inputs have a broad range of biotechnological and biomedical applications. Here we describe the design of switchable enzymes based on an ensemble allosteric model. First, we insert an enzyme domain into an effector-binding dom...

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Publicat a:Nat Commun
Autors principals: Choi, Jay H., Laurent, Abigail H., Hilser, Vincent J., Ostermeier, Marc
Format: Artigo
Idioma:Inglês
Publicat: 2015
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4704092/
https://ncbi.nlm.nih.gov/pubmed/25902417
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms7968
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