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Directed Evolution of a Highly Specific FN3 Monobody to the SH3 Domain of Human Lyn Tyrosine Kinase

Affinity reagents of high affinity and specificity are very useful for studying the subcellular locations and quantities of individual proteins. To generate high-quality affinity reagents for human Lyn tyrosine kinase, a phage display library of fibronectin type III (FN3) monobodies was affinity sel...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Huang, Renhua, Fang, Pete, Hao, Zengping, Kay, Brian K.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4701441/
https://ncbi.nlm.nih.gov/pubmed/26731115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0145872
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