Development of a Highly Automated and Multiplexed Targeted Proteome Pipeline and Assay for 112 Rat Brain Synaptic Proteins

We present a comprehensive workflow for large scale (>1000 transitions/run) label-free LC-MRM proteome assays. Innovations include automated MRM transition selection, intelligent retention time scheduling (xMRM) that improves Signal/Noise by >2-fold, and automatic peak modeling. Improvements t...

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Detalhes bibliográficos
Publicado no:Proteomics
Main Authors: Colangelo, Christopher M., Ivosev, Gordana, Chung, Lisa, Abbott, Thomas, Shifman, Mark, Sakaue, Fumika, Cox, David, Kitchen, Rob R., Burton, Lyle, Tate, Stephen A, Gulcicek, Erol, Bonner, Ron, Rinehart, Jesse, Nairn, Angus C., Williams, Kenneth R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4698340/
https://ncbi.nlm.nih.gov/pubmed/25476245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201400353
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