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An improved method for western blotting when extracting proteins from mammalian cells cultured on a collagen gel under serum-free conditions
Western blotting is a widely used method for detection and quantification of specific proteins extracted from mammalian cells. In the conventional method of protein extraction, we found that collagen-containing gels interfered with detection of the p65 protein (one of the subunits in the NF-κB famil...
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| Udgivet i: | Cytotechnology |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Springer Netherlands
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4698262/ https://ncbi.nlm.nih.gov/pubmed/25005915 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-014-9766-4 |
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