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Protocol to culture cells on genipin-mixed collagen gels with different stiffnesses

Summary: The stiffness of extracellular matrices is critical for cellular functions such as growth and differentiation. Here, we present a protocol for culturing cells on stiffness-modulated collagen gels by adding genipin, an amine crosslinker with low cytotoxicity. We describe the steps for prepar...

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Detalhes bibliográficos
Main Authors: Seiichiro Ishihara, Hisashi Haga
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2025-12-01
Colecção:STAR Protocols
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Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2666166725005313
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